云南主要核桃品种的ISSR分子鉴别

以核桃当年萌发的新叶为材料,在建立了良好的反应体系基础上,筛选出8条引物对云南省11个主要栽培和推广的核桃品种进行了ISSR分子标记研究。结果表明:8个ISSR引物共检测出位点102个,其中多态性位点62个,占60.78%,参试样品具有较高的多态比率;用其中的两条引物建立了11个品种的指纹图谱,并可用之鉴别参试品种;POPGENE 32软件的计算结果有效等位基因数、基因的多样性和Shannon信息指数分别为1.370 7,0.214 3和0.321 6,供试的云南核桃品种在分子水平上存在比较丰富的遗传变异。     

A new neuroprotective bakkenolide from the rhizome of Peatasites tatewakianus

Constituents of the fruits of Peatasites tatewakianus were investigated. A new compound, namely bakkenolide-VI (1), was isolated. The structures was elucidated on the basis of 1D, 2D NMR, TOF-MS and ESI-MS techniques, and physicochemical properties. The neuroprotective activity of the new compound was assayed with primary cultured neurons exposed to oxygen-glucose deprivation and oxidative insults.     

澳大利亚湿加松F2杂种引种试验结果初报

对种植于广东省蕉岭、四会、信宜、阳东等4个地点的澳大利亚湿加松F2杂种2．5～4．0年生的人工林作了生长量调查和数据分析，结果表明：F2杂种早期生长突出，其年均树高生长介于0．87—1．10m、年均胸径生长介于1．50～1．99cm，比同地点湿地松的树高、胸径分别大19．40％～26．64％，18．78％～47．48％，比马尾松的树高、胸径分别大3．28％～24．71％，42．45％～80．07％。初步认为，湿加松F2杂种在广东省的低山地区有较大的发展潜力。文章还对F2：杂种的变异程度进行了探讨，并提出了F2杂种的利用设想。     

Preliminary studies on the early quality identification ofAuricularia auricular

In production industry ofAuricularia auricular, the varieties quality is most important impact factor on output. For evaluating the early quality of the edible fungus, 9 varieties ofAuricularia auricular (Au9, CF09, CF05, 29, 916, chang10, chang7, Au.Japanese and 8808) were cultured on the medium consists of potato dextrose agar (PDA) and sawdust to test their mycelium growth rate, endurance to high temperature, resistance to mildew, assimilation of nutriment, and resistance to drought. The result showed that the mycelium of Chang 10, CF09, 29 and Au.Japanese varieties had the eminent characteristics such as short lifespan, stronger assimilation of nutriment, and endurance to high temperature and steady growth. These four varieties are determined as superiority varieties ofAuricularia auricular in accordance with the research results. Foundation item: The research was supported by Science Fund of Northeast Forestry University (2004). Biography: Li Ling (1964-), female, Engineer in Northeast Forestry University, Harbin 150040, P. R. China. Responsible editor: Zhu Hong     

GPS在林业面积测量中的应用研究

利用两台GPS进行面积测量,将所采集的数据与复枧法罗盘仪闭合导线测量的数据进行对照分析。分析结果认为:在面积3.4hm~2以上时精度可达90％,在面积为8.2hm~2时精度可达95％。     

红刺玫染色体的观察和核型分析

作者主要对红刺玫细胞染色体计数,并对其核型进行分析。研究结果表明:红刺玫的染色体为三倍体,2n=3x=21;核型公式为2n=3x=21=18m 3sm,属于2A型。     

一种适合杨树RNA提取的方法

本试验为三种杨树材料建立了一种杨树RNA提取的方法。本方法具有充分抑制材料中的多酚褐化,提高RNA产率;最大限度地去除多糖及次生代谢物质,避免与RNA共沉淀;去除蛋白质和DNA污染;提取时间短,质量高等特点。通过本方法提取的RNA OD260/OD280值在1.9~2.0之间,能够完全满足后续实验的要求,如体外翻译、cDNA文库构建、基因芯片和DDRT-PCR等。     

水曲柳无性系初级种子园营建技术标准

在种源选择和子代测定,并吸收前期水曲柳种子园营建技术成果、文章的基础上,参考国内外其它树种种子园营建技术、国家地方相关标准、规程和有关政策,对水曲柳无性系种子园的规划设计、建园及经营管理技术进行了明确界定与规范,制定水曲柳无性系种子园营建技术标准,旨在为生产部门建立水曲柳无性系种子园提供技术支撑。     

森林游憩开发中的盈利模式研究

森林游憩的深度开发 ,呼唤盈利模式的研究与运用。尝试提出森林游憩开发的技术路线 ,并对其开发的盈利模式进行探讨。